Journal: The Journal of Neuroscience

Article Title: Ablation of Retinal Horizontal Cells from Adult Mice Leads to Rod Degeneration and Remodeling in the Outer Retina

doi: 10.1523/JNEUROSCI.0442-12.2012

Figure Lengend Snippet: Primary and secondary antibodies used in this study

Article Snippet: mGluR6 , Rabbit anti-mGluR6 , Acris Antibodies , 1:1000.

Techniques: Recombinant

Journal: The Journal of Neuroscience

Article Title: Ablation of Retinal Horizontal Cells from Adult Mice Leads to Rod Degeneration and Remodeling in the Outer Retina

doi: 10.1523/JNEUROSCI.0442-12.2012

Figure Lengend Snippet: Ectopic synapses in retinae of horizontal cell-ablated mice. A, B, E, F, In treated control retinae, PKCα-positive dendrites of rod bipolar cells (green) terminated within the PSD-95-labeled rod spherules in the OPL (magenta; B, arrowhead). In Cx57+/DTR retinae, extending bipolar cell dendrites occasionally contacted retracting PSD-95-positive rod spherules in the ONL (F, arrowheads). C, D, G, H, Triple staining with antibodies against PKCα for rod bipolar cells (blue), CtBP2 for synaptic ribbons (magenta), and mGluR6 (green), the glutamate receptor subunit of ON bipolar cells. In controls, ribbons were always closely associated with mGluR6-positive tips on rod bipolar cell dendrites (D, arrowhead). In Cx57+/DTR retinae, the typical horseshoe-shaped structure of the ribbons was lost, but CtBP2 immunosignals were still present within the ONL and OPL. Also, in the ONL, we found sprouting dendrites decorated with mGluR6 puncta in close vicinity to CtBP2-positive ribbons (H, arrowheads). These structures were presumably ectopic synapses. I, J, A putative ectopic synapse in the ONL of a Cx57+/DTR retina 6 months after DT treatment. A bipolar cell dendrite contacted a vesicle-filled photoreceptor terminal (shorter arrows), in which a single synaptic ribbon is visible (long arrow). K, Horizontal cell-ablated mice showed ectopic ribbons surrounded by vesicles (long arrow) also close to the outer limiting membrane (short arrows). Scale bars: A–C, E–G, 10 μm; D, H, 5 μm; I, K, 1 μm; J, 250 nm.

Article Snippet: mGluR6 , Rabbit anti-mGluR6 , Acris Antibodies , 1:1000.

Techniques: Labeling, Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Characterization of neurite outgrowth and ectopic synaptogenesis in response to photoreceptor dysfunction

doi: 10.1007/s00018-012-1230-z

Figure Lengend Snippet: Outgrowing horizontal cells and the formation of ectopic synapses. Confocal images of the outer retina of A3B1 mice at p18 (a), p28 (b), P36 (c), and P43 (d) double labeled for calbindin (green) and the presynaptic ribbon marker CtBP2 (magenta). Arrows in b mark the first ectopic appearing synapses in the ONL. Magnification view of a horizontal cell process tip in the ONL directly surrounded by a horseshoe-shaped ribbon is shown in the inset in c. d At P43, all aberrant horizontal cell processes were associated with ectopic synapses at their tips (arrows), some processes were flanked by ectopic synapses along the shaft (arrowheads). e–g Rod bipolar cell (RBC) outgrowth in the A3B1 mouse at different time points. e RBC staining (PKCα, magenta) reveals no sprouting at P18. f, g Double labeling of HCs (Calbindin, green) and RBCs (PKCα, magenta) reveals that the aberrant processes from these two cell types frequently exit the OPL at the same points and navigate together through the ONL. The arrow in f marks a RBC dendrite on the way along the same path as the HC process. h Double labeling of PKCα (magenta) and mGluR6 (green) reveals ectopic mGluR6 expression on sprouting RBC dendrites. Scale bars 10 μm in b (for a–d), in e (for e–g) and h

Article Snippet: Other antibodies used were: guinea pig anti-mGluR6 (1:200, GP13105, Neuromics, Edina, MN, USA), goat anti-Lgals3 (1:300, sc-19283, Santa Cruz Biotechnologies, Heidelberg, Germany), rat anti-Plxnc1 (1:200, M652, Amgen, Seattle, WA, USA) [ 17 ], rabbit anti-phospho-Smad1/5/8 (1:300, #9511, Cell Signaling Technology), rabbit anti-phospho-Src (Tyr416) (1:20, #2101, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Stat3 (Tyr705) (1:100, #9145, Cell Signaling Technology) and rabbit anti-TGFβ1 (1:200, G122A, Promega, Mannheim, Germany).

Techniques: Labeling, Marker, Staining, Expressing

Journal: iScience

Article Title: A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation

doi: 10.1016/j.isci.2021.103657

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mGluR6 , Novus Biologicals , Cat#NLS4655 RRID: AB_343723.

Techniques: Control, Recombinant, Knock-Out, Software, Imaging